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中华乳腺病杂志(电子版) ›› 2014, Vol. 08 ›› Issue (05) : 19 -23. doi: 10.3877/cma. j. issn.1674-0807.2014.05.005

论著

叉头框转录因子M1、Slug、E-cadherin 及vimentin在基底细胞样乳腺癌中的表达及其临床意义
周炳娟, 马秋双, 陈红, 张金库()   
  • 收稿日期:2014-03-17 出版日期:2014-10-01
  • 通信作者: 张金库
  • 基金资助:
    河北省卫生厅青年课题项目(20130333)

Expression of forkhead box M1, Slug, E-cadherein and vimentin in basal-like breast carcinoma and their clinical significance

Bingjuan Zhou, Qiushuang Ma, Hong Chen, Jinku Zhang()   

  • Received:2014-03-17 Published:2014-10-01
  • Corresponding author: Jinku Zhang
引用本文:

周炳娟, 马秋双, 陈红, 张金库. 叉头框转录因子M1、Slug、E-cadherin 及vimentin在基底细胞样乳腺癌中的表达及其临床意义[J]. 中华乳腺病杂志(电子版), 2014, 08(05): 19-23.

Bingjuan Zhou, Qiushuang Ma, Hong Chen, Jinku Zhang. Expression of forkhead box M1, Slug, E-cadherein and vimentin in basal-like breast carcinoma and their clinical significance[J]. Chinese Journal of Breast Disease(Electronic Edition), 2014, 08(05): 19-23.

目的

探讨叉头框转录因子M1(FOXM1)、Slug 及上皮间质转化的相关蛋白E-钙黏蛋白(E-cadherin)、波形蛋白(vimentin)在基底细胞样乳腺癌(BLBC)、非基底细胞样乳腺癌(non-BLBC)及癌旁乳腺组织中的表达及其临床意义。

方法

采用免疫组织化学MaxVision 法对2005 年1 月至2014 年5 月在本院治疗的95 例BLBC 及其癌旁乳腺组织和100 例non-BLBC 中FOXM1、Slug、E-cadherin 及vimentin 的表达进行检测。 计数资料的分析运用χ2 检验,相关性分析采用Spearman 相关性分析。

结果

在95 例BLBC 中,FOXM1、Slug 和vimentin 蛋白的阳性表达率分别为76.84%(73/95)、67.36%(64/95)及52.63%(50/95),显著高于其在non-BLBC[57.00%(57/100)、47.00%(47/100)、31.00%(31/100)]及癌旁乳腺组织[25.26% (24/95)、26.31% (25/95)、9.47% (9/95)]中的表达(χ2 =51.673、32.146、41.337, P 均=0.000)。 BLBC 中E-cadherin 的阳性表达率为27.37%(26/95),显著低于其在non-BLBC[51.00%(51/100)]及癌旁乳腺组织[75.79%(72/95)]中的表达(χ2 =44.590,P=0.000)。FOXM1、Slug、E-cadherin 及vimentin 的表达均与BLBC 的淋巴结转移及临床分期有关,差异具有统计学意义(χ2=4.348、4.669、18.387、21.287,P 均<0.050; χ2 =7.752、5.366、4.230、7.167,P 均<0.050)。BLBC 中,FOXM1、Slug 与vimentin 的表达呈正相关(r=0.279,P=0.006;r=0.284, P=0.005),与E-cadherin 表达呈负相关(r=-0.503, P=0.000; r=-0.378, P=0.000), FOXM1 与Slug 的表达呈正相关(r=0.310, P=0.002)。 E-cadherin 与vimentin 的表达呈负相关(r=-0.363, P=0.000)。

结论

FOXM1及Slug 通过抑制E-cadherin 表达、促进vimentin 表达从而促进了BLBC 的局部侵袭和远处转移,可以作为BLBC 预后评价的指标。

Objective

To detect the expression and clinical significance of forkhead box M1(FOXM1), Slug, E-cadherin and vimentin (the last two were epithelial-mesenchymal transition associated proteins) in basal-like breast cancer (BLBC).

Methods

The expressions of FOXM1, Slug, E-cadherin and vimentin were analyzed in 95 cases of BLBC, 95 cases of adjacent breast tissue and 100 cases of non-BLBC treated in our hospital from January 2005 to May 2014 by immunohistochemistry. The enumeration data were analyzed by χ2 test. The Spearman correlation analysis was performed.

Results

The positive expression rate of FOXM1, Slug and vimentin in BLBC was 76.84% (73/95), 67.36% (64/95) and 52.63% (50/95)respectively, which was significantly higher than that in non-BLBC [57.00% (57/100),47.00% (47/100),31.00% (31/100)] or adjacent breast tissue [25.26% (24/95),26.31% (25/95),9.47% (9/95)] (χ2=51.673,32.146,41.337,all P=0.000). The positive rate of E-cadherin in BLBC was 27.37% (26/95),much lower than that in non-BLBC [51.00% (51/100)] and adjacent breast tissue [75.79% (72/95)](χ2=44.590,P=0.000). The expressions of FOXM1, Slug, E-cadherin and vimentin were correlated with lymph metastasis and pTNM staging in BLBC patients (χ2=4.348, 4.669, 18.387, 21.287,P<0.050; χ2=7.752,5.366,4.230, 7.167,P <0.050). In BLBC patients, the expression of FOXM1 and Slug had a positive correlation with that of vimentin (r=0.279,P=0.006;r=0.284, P=0.005) and had a negative correlation with that of E-cadherin (r=-0.503, P=0.000; r=-0.378, P=0.000). There was a positive correlation between FOXM1 and Slug (r=0.310, P=0.002) and a negative correlation between E-cadherin and vimentin (r=-0.363, P=0.000).

Conclusion

FOXM1 and Slug may play an important role in the invasion and metastasis of BLBC by downregulation of E-cadherin and upregulation of vimentin synergistically,which can be used as prognostic factors of BLBC.

表1 BLBC、 non-BLBC 和癌旁乳腺组织中FOXM1、Slug、E-cadherin 和vimentin 的表达
表2 基底细胞样乳腺癌临床病理特征与FOXM1、Slug、E-cadherin 和vimentin 表达的关系
表3 FOXM1 和E-cadherin、Slug、vimentin 在基底细胞样乳腺癌中表达的相互关系
表4 Slug 和E-cadherin、vimentin 在基底细胞样乳腺癌中表达的相互关系
表5 E-cadherin 和vimentin 在基底细胞样乳腺癌中表达的相互关系
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